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. 1999 Aug 1;104(3):309–316. doi: 10.1172/JCI7016

Figure 7.

Figure 7

Positive-feedback loop in hBLTR TG mice. 5-LO transcripts and product (5-HETE) are upregulated in peritonitis. (a) MS/MS spectrum of 5S-HETE. Peritoneal leukocytes from casein-induced peritonitis were collected and analyzed by LC/MS/MS. SIM chromatograms at m/z 319, as well as MS/MS spectra of 5-HETE, were acquired with a Finnigan LCQ LC/MS/MS system. 5-HETE was identified by its diagnostic MS/MS product ions at m/z 319 (A), 301 (A-H2O), 275 (B), 257 (B-H2O), and 115 (C). (b) Levels of 5-HETE, 15-HETE, and 12-HETE in nTG (hatched bars) and hBLTR TG mice (filled bars) were quantitated by LC/MS/MS and expressed in picograms per 5 × 106 peritoneal exudate leukocytes (*P < 0.01; n = 3). (c) Total RNA from isolated exudate leukocytes of nTG and hBLTR TG mice was isolated and analyzed for 5-LO and GAPDH expression by RT-PCR. The expected PCR product for 5-LO at approximately 0.3 kb was indicated by an arrow, and the relative intensities of RT-PCR products (5-LO/GAPDH) were determined.