Figure 3.

TLC immuno-overlay of mAb’s cloned from mice immunized with LPS. The resorcinol-stained panels reveal individual ganglioside mobility, and the schema refers to both a and b. All IgM mAb’s (a) react strongly with GD3, and most also react with GQ1b and GT1a gangliosides. However, subtle differences in epitope specificity and intensity are evident. The IgG mAb’s (b) react more selectively with GD3 only, and in the case of EG1, with GD3 and GQ1b. TLC immuno-overlay of LPSs (c) demonstrates selective reactivity of the mAb CGM3 with the immunizing GT1a-like OH4384 LPS and the structurally similar GD3-like OH4382 LPS, but not with the GM1-like O:19 serostrain LPS.