Fig. 3.

Expression analysis of the sam8 transformant. (A) RT-PCR analysis of the sam8 Synechocystis 6803transformant. (Upper) PCR analysis using primers that bind to sam8. (Upper) PCR analysis using primers that bind to cDNA from 16S rRNA, which was used as a loading control. Lanes: M, DNA ladder; dH₂O, negative control for PCR using water as template; WT+RT: RT-PCR using RNA from wild-type Synechocystis 6803 as template for the reverse transcription; WT-RT: as for WT+RT but with no RT in the reaction; sam8+RT: RT-PCR using RNA from the Synechocystis 6803 mutant transformed with sam8 as template for the reverse transcription; sam8-RT: as for sam8+RT, but with no RT in the reaction. (B) SDS/PAGE of the sam8 Synechocystis 6803 transformant. Total cell extract of wild-type Synechocystis 6803 and the sam8 transformant were fractionated by SDS/PAGE and stained with Coomassie blue. Lanes: WT, crude extracts from wild-type Synechocystis 6803; sam8, crude extracts from the sam8-expressing Synechocystis 6803 transformant. TAL is labeled. (C) Western blot analysis of the sam8 Synechocystis 6803 transformant. The proteins in B were blotted onto a nitrocellulose membrane and probed with anti-TAL antibody.