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. 2014 Jun 9;111(26):9449–9454. doi: 10.1073/pnas.1323725111

Fig. 5.

Fig. 5.

Functional analysis of slr1573 in E. coli. (A) Plasmid construction for laccase expression in E. coli. The slr1573 gene was inserted into pET-28(+) at the multiple cloning sites between BamHI and SacI. (B) Laccase expression in E. coli. Putative laccase proteins are indicated by an arrow. Samples were collected at 0 h, 1 h, 2 h, 3 h, and 4 h after IPTG induction. P1: proteins in cell extract were purified by affinity chromatography; P2: proteins from P1 were treated by thrombin to remove the associated His tags. Calculated molecular mass of His-laccase: 32.66 kDa. (C) Laccase activity of cell extracts from E. coli harboring slr1573. Error bars represent 1 SD for triplicate assays.