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. 2014 Jun 16;111(26):9461–9466. doi: 10.1073/pnas.1403101111

Fig. 2.

Fig. 2.

ODA-DC is a heterotrimer. (A) Sedimentation coefficient distribution function c(s) was obtained from sedimentation velocity data by SEDFIT (36). c(s) was converted to c(M) to estimate the molecular mass of DC1–2–3 to be ∼152 kDa. (B) There were two minor peaks in A; 2.74% of the solutes had an S value of 3.04S (64.5 kDa), which is close to the molecular mass of DC2 (62,204 Da). This peak most likely reflects DC2–6×His that was purified by Ni⋅NTA but did not form a complex with DC1 or DC3; 0.43% of the solutes had an S-value of 8.91S (325 kDa), which is close to twice the sum of the molecular masses of DC1, DC2, and DC3 (333,850 Da). This peak suggests that a small fraction of the DC1–2–3 complexes formed dimers.