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ePTEN-GFP (PTENQ17R, R41G, E73D, N262Y, N329H-GFP) is highly enriched at the plasma membrane in HEK293T cells. (A) HEK293T cells expressing the indicated forms of PTEN-GFP were observed by fluorescence microscopy. (Scale bar, 20 µm.) (B and C) Intensity of GFP at the plasma membrane was quantified relative to that in the cytosol. Values represent the mean ± SD (n ≥ 15). The values for PTENR41G, E73D, N262Y, N329H and PTENQ17R, R41G, E73D, N262Y, N329H are duplicated in B and C.
