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. 2014 Jun 16;111(26):E2684–E2693. doi: 10.1073/pnas.1409433111

Fig. 6.

Fig. 6.

ePTEN-GFP (PTENQ17R, R41G, E73D, N262Y, N329H-GFP) does not bind to the inhibitory C-terminal tail. (A) Interaction of the C-terminal domain of PTEN with its N-terminal core region was assessed in pull-down assays. PTEN352–403-YFP-FLAG was added to whole-cell lysates expressing the indicated PTEN-GFP constructs and immunoprecipitated with beads coupled to anti-FLAG antibodies. Bound fractions (IP) were analyzed with antibodies to GFP and FLAG. (B) Band intensity was quantified (n = 3). (C) Whole-cell lysates expressing different PTEN-GFP constructs were analyzed by immunoblotting with antibodies against phospho-PTEN (pS380) and GFP (PTEN-GFP). (D) Band intensity was quantified (n ≥ 3). (E) The indicated PTEN-GFP proteins were immunopurified from Dictyostelium cells, and phosphatase activities were measured (n ≥ 3).