Skip to main content
. 2014 Jun 9;111(26):9591–9596. doi: 10.1073/pnas.1407473111

Fig. 2.

Fig. 2.

Generation of the seamless CCR5∆32 mutation by piggyBac transposase excision. (A) PCR analyses after transposon excision. (Top) Absence of a band indicates biallelic excision of the transposon at the CCR5 locus using P2 and P5 primers. (Middle) Presence of the Δ32 band indicates successful generation of the 32-bp deletion using P3 and P4 primers. (Bottom) Absence of a band indicates complete removal of transposon from the genome, using P1 and P6. (B) Sequence analyses for seamless excision of transposon. (Upper) Sequences of excision clone reveals a 32-bp deletion before the TTAA site. (Lower) Alignment of excision clone (T1-6-6) sequences with wild type and Δ32 mutation of the CCR5 gene.