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. Author manuscript; available in PMC: 2015 May 26.
Published in final edited form as: Chembiochem. 2014 Apr 24;15(8):1101–1105. doi: 10.1002/cbic.201400032

Scheme 1.

Scheme 1

Sulfatase-catalyzed hydrolysis of MFS and RS forms bright fluorescent products. MFS is non-fluorescent before hydrolysis to give MF, a product with a fluorescence quantum yield (Φ) of 0.45. RS is effectively non-fluorescent (Φ ≤ 0.01) before hydrolysis to produce resorufin (Φ = 0.74).