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. Author manuscript; available in PMC: 2015 May 26.
Published in final edited form as: Chembiochem. 2014 Apr 24;15(8):1101–1105. doi: 10.1002/cbic.201400032

Table 1.

Kinetic parameters for MFS.

Enzyme Source Aerobacter
aerogenes[a]
Helix
pomatia[b]
Abalone
Entrails[b,c]
KM (µM) 598 ± 67 24.8 ± 2.2 1070 ± 420
Vmax (pmoles/sec) 1.25 ± 0.08 6.9 ± 0.2 10.4 ± 3.3
Vmax/KM (nL/sec) 2.10 277 9.74
[a]

Assay was performed in Tris buffer (pH 7.5) at 37 °C.

[b]

Assay was performed in potassium acetate buffer (pH 5.0) at 37 °C.

[c]

Inhibition was observed (Ki = 770 ± 370 µM).