Table 1.
Enzyme Source |
Aerobacter aerogenes[a] |
Helix pomatia[b] |
Abalone Entrails[b,c] |
---|---|---|---|
KM (µM) | 598 ± 67 | 24.8 ± 2.2 | 1070 ± 420 |
Vmax (pmoles/sec) | 1.25 ± 0.08 | 6.9 ± 0.2 | 10.4 ± 3.3 |
Vmax/KM (nL/sec) | 2.10 | 277 | 9.74 |
Assay was performed in Tris buffer (pH 7.5) at 37 °C.
Assay was performed in potassium acetate buffer (pH 5.0) at 37 °C.
Inhibition was observed (Ki = 770 ± 370 µM).