Figure 6.

Nitrate/nitrite determination in 12-LO KO and C57BL/6 peritoneal macrophages. Peritoneal macrophages were purified as described in Methods and stimulated with LPS (2 μg/mL) + IFN-γ (100 ng/mL) for 48 hours. We used Griess reagent to measure total medium nitrate/nitrite as an estimate of NO production. As shown, 12-LO KO macrophages generated ∼50% of the NO of C57BL/6 macrophages under the conditions described. Six individual experiments were performed with macrophages isolated from both strains of mice. *P < 0.0001 vs. C57BL/6 macrophages.