Figure 1. Phenotype of Miz1^ΔPOZNes mice.

(a) Miz1^ΔPOZNes mice move slowly and lack motor co-ordination. The panel shows the average speed of movement and time on a rotarod (maximum: 300 sec) maintained at constant speed of control and age-matched Miz1^ΔPOZNes mice. The average age of mice tested was 16 months. p-values were calculated using an unpaired two-tailed Student's t-test (The number of animals tested is shown below each graph).

(b) Defect of cerebellar growth in Miz1^ΔPOZNes mice. The panels show sagittal sections of brains of control and Miz1^ΔPOZNes mice at the indicated age. Dotted lines show the circumference of the cerebellum. For each mouse, the cerebellar surface area was determined; numbers show the ratio between control and Miz1^ΔPOZNes mice. The number of mice analyzed for each genotype is indicated on the right. Scale bar: 1mm.

(c,d): Histology of the cerebellum. Sections of the cerebellum of 11 month-old control (c) and Miz1^ΔPOZNes (d, please note different scale) mice were stained with antibodies directed against calbindin, a marker for Purkinje cells, and GFAP, a marker for Bergman glial cells. Boxes indicate the area of a detail picture in the overviews. GFAP staining is shown for adjacent section (arrowheads: regions of missing Purkinje cells). Scale bar: Upper panels: 500 μm, lower panels: 100 μm.

(e) Enhanced apoptosis in the granular layer of cerebella in Miz1^ΔPOZNes mice. The upper panel shows representative TUNEL staining of cerebella of age-matched control and Miz1^ΔPOZNes mice. The lower panel shows a quantification of staining observed in four control and five Miz1^ΔPOZNes mice. Error bars show standard error of the mean (SEM). p-value was calculated using an unpaired two-tailed Student's t-test.