Figure 3.

Flow cytometry analysis of thymocytes from E17.5 and E18.5 FTOCs treated with selective COX-1 inhibitor. Thymocytes from E17.5 plus 5 days culture in FTOCs were examined for CD4 and CD8 expression. (a) Vehicle. (b) L-759,700, 10 μM. The percentage of cells in each quadrant are indicated in the corners of each graph. (c) E15.5 (filled circles), E17.5 (filled squares), and E18.5 (filled triangles) FTOCs were treated with different concentrations of L-759,700. The percent inhibition of the DP absolute counts is expressed relative to vehicle-treated FTOCs. Values are means ± SD of 3–6 different experiments, each performed in quadruplicate.