Table 1.
Oligonucleotide sequences
| Name | Sequence (5’-3’) |
|---|---|
| NF-κB-sence |
CGGAATTCGCCACCAGAATGGCAGAAGATGATC |
| NF-κB-antisence |
TGTCACTCGAGGCAATTTTGCCTTCTAGAGGTC |
| NF-κB-siR-Top |
GATCCCGCCTGAACAAATGTTTCATTTGGTCAAGAGCCAAATGAAACATTTGTTCAGGCTTTTTTGGAAA |
| NF-κB-siR-Bot |
AGCTTTTCCAAAAAAGCCTGAACAAATGTTTCATTTGGCTCTTGACCAAATGAAACATTTGTTCAGGCGG |
| Pri-130a-sense |
ATGGGATCCAGAGGGAGCCCGTGAGCTG |
| Pri-130a-antisense |
CGGAATTCGTATAACTAACAAGTGAGGCTACC |
| ASO-miR-130a |
AUGCCCUUUUAACAUUGCACUG |
| ASO-NC |
TGACTGTACTGAGACTCGACTG |
| miR-130a-RT |
GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACATGCCCT |
| miR-130a-Forward |
TGCGGCAGTGCAATGTTA |
| U6-RT |
GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATGG |
| U6- Forward |
TGCGGGTGCTCGCTTCGGCAGC |
| miR-Reverse primer |
CCAGTGCAGGGTCCGAGGT |
| TNF-α-3’UTR-sense |
CGGGATCCGAAATTGACACAAGTGGACC |
| TNF-α-3’UTR-antisense |
CGGAATTCCTCCCAAATAAATACATTCATCTG |
| TNF-α-3’UTR-mut-sense |
CCCTCTATTTATGATACGAGTTGTGATTATTT |
| TNF-α-3’UTR-mut-antisense |
AAATAATCACAACTCGTATCATAAATAGAGGG |
| TNF-α-sense |
CGGAATTCGCCACCATGAGCACTGAAAGC |
| TNF-α-antisense |
CCCGCTCGAGGCCAGGGCAATGATCCCAAAG |
| β-actin-sense |
CGTGACATTAAGGAGAAGCTG |
| β-actin-antisense |
CTAGAAGCATTTGCGGTGGAC |
| GAPDH-sense |
GCGAATTCCGTGTCCCCACTGCCAACGTGTC |
| GAPDH-antisense | GCTACTCGAGTTACTCCTTGGAGGCCATGTGG |