Table 3.

The effect of tau phosphorylation on cell death in the presence of model Hirano bodies

Sample	%Cell death
Hirano body/GSK3β(K85A)	4.20 ± 0.77
Hirano body/G272V	17.90 ± 2.59
Hirano body/G272V/GSK3β(K85A)	10.20 ± 1.35
GFP/G272V	4.75 ± 0.74
GFP/G272V/ GSK3β(K85A)	5.80 ± 0.56
GFP/G272V + GFP/ GSK3β(K85A)	8.95 ± 2.06

Expression of GSK3β(K85A) reduced the synergistic cell death observed when both G272V and model Hirano bodies are present, to an additive effect, implying that the phosphorylation state is important.