Table 3. Primer sequences.
A list of RT-PCR primer sequences of utilized to assess sWAT derived from WT mice versus MitoN-Tg mice.
| Gene | Forward primer | Reverse primer |
|---|---|---|
| β-actin | TACCACAGGCATTGTGATGG | TTTGATGTCACGCACGATTT |
| MitoNEET | ATGCCTTCGACATGGAGGATCT | TCGTTGTGCTTTATGTGAGCCC |
| Pgc1α | TCCTCACACCAAACCCACAGAA | TTGGCTTGAGCATGTTGCGA |
| Ucp1 | GTGAAGGTCAGAATGCAAGC | AGGGCCCCCTTCATGAGGTC |
| F4/80+ | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Cd206 | TGTGGTGAGCTGAAAGGTGA | CAGGTGTGGGCGCAGGTAGT |
| Cd301 | TGAGAAAGGCTTTAAGAACTGGG | GACCACCTGTAGTGATGTGGG |
| IL-10 | GCTCTTACTGACTGGCATGAG | CGCAGCTCTAGGAGCATGTG |
| Cd163 | TCCACACGTCCAGAACAGTC | CCTTGGAAACAGAGACAGGC |
| Mcp-1 | ACTGAAGCCAGCTCTCTCTTCC | TTCCTTCTTGGGGTCAGCACAG |
| IL-6 | CCAGAGATACAAAGAAATGATGG | ACTCCAGAAGACCAGAGGAAAT |
| FoxP3 | TCAAGTACCACAATATGCGACC | CCATCGGATAAGGGTGGCA |
| Tbet | GCCAGGGAACCGCTTATATG | GACGATCATCTGGGTCACATTGT |
| Vegf-A | GGAGATCCTTCGAGGAGCACTT | GGCGATTTAGCAGCAGATATAAGAA |
| Vegf-R | TGCCTACCTCACCTGTTTCC | AAGGACCATCCCACTGTCTG |
| Tie2 | AAGCAACCCAGCCTTTTCTC | TGAGCATTCTCCTTTGGAC |
| Elastin | TGGTATTGGTGGCATCGG | CCTTGGCTTTGACTCCTGTG |
| Cd31 | ATGACCCAGCAACATTCACA | CGACAGGATGGAAATCAACAA |
| Hif1α | CAAGATCTCGGCGAAGCAA | GGTGAGCCTCATAACAGAAGCTTT |
| Flt-1 | TGCCTACCTCACCTGTTTCC | AAGGACCATCCCACTGTCTG |
| Col6α1 | GATGAGGGTGAAGTGGGAGA | CAGCACGAAGAGGATGTCAA |
| Col3α1 | GGGTTTCCCTGGTCCTAAAG | CCTGGTTTCCCATTTTCTCC |
| Col1α1 | GTGCTCCTGGTATTGCTGGT | AAGGACCATCCCACTGTCTG |
| Cd36 | TGAGACTGGGACCATTGGTGAT | CCCAAGTAAGGCCATCTCTACCAT |
| Fabp4 | GACGACAGGAAGGTGAAGAG | ACATTCCACCACCAGCTTGT |
| Fas | CCTGGATAGCATTCCGAACCT | AGCACATCTCGAAGGCTACACA |
| Fasn | AAGTTGCCCGAGTCAGAGAA | CGTCGAACTTGGAGAGATCC |
| Pparγ | TCAGAGGGACAAGGATTCATGA | CACCAAAGGGCTTCCGCAGGCT |
| Glut4 | AAGATGGCCACGGAGAGAG | GTGGGTTGTGGCAGTGAGTC |
| Pepck | CGCAAGCTGAAGAAAATATGACAA | TCGATCCTGGCCACATCTC |