Figure 1. Induction of intestinal Th17 cells by SFB requires MHCII expression in the periphery.

A. Th17 and Treg cell proportions in SI LP of SFB-positive WT and MHCII-deficient (IAb^−/−) mice. Foxp3 and cytokine staining plots are gated on TCRβ⁺CD4⁺ cells

B. Th17 and Treg cell proportions in SI LP of SFB-negative (Jackson microbiota) and SFB-positive (Taconic microbiota) WT and IAb^−/− mice. Plots gated on TCRβ⁺CD4⁺ cells

C–D. WT CD45.1⁺ CD4 T cells were adoptively transferred into WT CD45.2 mice before or 12 days after SFB colonization. Cytokine expression in host (CD45.2⁺) and donor (CD45.1⁺) SI LP TCRβ⁺CD4⁺ cells 2 weeks after transfer. Data from one of multiple experiments

E–F. Th17 cell induction in WT CD4 T cells two weeks after transfer into SFB-positive WT and MHCII-deficient recipients. Plots gated on TCRβ⁺CD4⁺ cells. Data from one of multiple experiments