T cell hybridomas were generated from SI LP GFP+ (Th17) and GFP− (non-Th17) CD4 T cells from SFB-positive Il17GFP mice. Data combined from 2 independent experiments
A. Number of hybridomas responding to SFB
B. Response of individual hybridomas (percentage of maximum anti-CD3 and anti-CD28 stimulation) to SFB or E. coli antigens as assessed by IL-2 production. Clones were ordered in decreasing amounts of IL-2 production