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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: DNA Repair (Amst). 2013 Apr 24;12(6):403–413. doi: 10.1016/j.dnarep.2013.03.003

Fig. 4. Binding of SsoRal1 and SsoRadA to ssDNA is influenced by nucleotide cofactors.

Fig. 4

EMSAs are shown for SsoRal1 in (A) and wild-type SsoRadA (B) where binding reactions were performed using ATP, ADP, or ATPγS. All reactions included 5 μM ssDNA and 100 mM NaCl. ATP and ADP concentrations were 3 mM, while ATPγS was present at a concentration of 50 μM. All wild-type SsoRadA reactions were cross-linked by the addition of glutaraldehyde to a final concentration of 0.75%. The position of the wells and unbound oligonucleotide are indicated.