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ENERGI-F704 activates AMPK in microglial BV2 cells. BV2 cells were treated with serial concentrations of ENERGI-F704 for 3 h. Cell lysates were used to determine the phosphorylation of AMPK using Western blot analysis. Data are presented as the mean ± SEM of three independent experiments (one-way ANOVA; **, P < 0.01; specific comparison to vehicle treated control). Representative images of three independent experiments are shown.
