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ENERGI-F704 increases the level of NAD in BV2 cells. BV2 cells were incubated with 200 ng/mL LPS in the presence of ENERGI-F704 or compound C for 24 h. Cell lysates collected from each condition were used to determine the level of NAD using NAD/NADPH colorimetric analysis. Data are presented as the mean ± SEM of three independent experiments (one-way ANOVA; *, P < 0.05).
