Figure 2.

In situ hybridization of wild-type embryos at E3.5 and E7.5. The embryos were stained with hematoxylin and eosin and hybridized with a probe complementary to the 3′ end of the mouse Ron transcript. Two distinct orientations of the blastocysts are shown: (a) orientation highlighting the trophectoderm of the blastocysts, and (b) orientation highlighting the inner cell mass. The light-field exposure is shown in the left panels, and the dark-field exposure is shown in the right panels. The bottom panel shows the dark-field exposure of a wild-type embryo isolated with the intact decidua at E7.5. TPHD, trophectoderm. ICM, inner cell mass. EE, extraembryonic tissue. E, embryonic tissue.