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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Sep 27;32(12):2974–2980. doi: 10.1161/ATVBAHA.112.300054

Figure 3.

Figure 3

Tumor necrosis factor α (TNFα)-induced protein kinase C (PKC) ζ signaling cascade depends on the p62 association with PKCζ. A, Human umbilical vein endothelial cell (HUVEC) was transfected with 80 nmol/L of either scrambled or p62 small interfering RNA (siRNA) (J-010230-05) and stimulated with TNFα (10 ng/mL) for the indicated times. Phosphorylation of JNK at Thr183/Tyr185 was analyzed by immunoblotting. B, Quantification of JNK phosphorylation that was normalized to the expression of total JNK. *P<0.01 compared with the respective time control. C, HUVEC was transfected with 80 nmol/L either scrambled or p62 siRNA (J-010230-05) and stimulated with TNFα (10 ng/mL)+cyclohexamide (CHX) (10 μg/mL) for 6 hours. The cleavage of caspase 3 was analyzed by immunoblotting. D, Quantification of cleaved form of caspase 3 that was normalized to the expression of actin. *P<0.01.