Skip to main content
. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Eur J Immunol. 2006 Aug;36(8):2170–2180. doi: 10.1002/eji.200635982

Fig. 4.

Fig. 4

Peripheral blood and liver NK cells express rNKp30. (A) F344 rat PBMC and liver mononuclear cells were analyzed by flow cytometry for rNKp30 (left columns, open histograms) or rNKp46 (right columns, open histograms) using CLH-9 or WEN23 mAb, respectively. Isotype controls (filled histograms) and PE-conjugated secondary reagents were used. Gates were set on NK cells (NKRP1-FITChi αβTCR-PerCP−, upper panels) and T cells (αβTCR-FITC+, lower panels). Representative data from three to four separate experiments are shown. (B) The proportion of rNKp30+ cells in the total NK cell population was quantitated (n=3). rNKp30+ NK cells comprise 63.1 ± 4.4% of spleen NK cells, 24.6 ± 7.2% of peripheral blood NK cells, and 14 ± 15.5% of liver NK cells. (C) PBMC (left) and liver mononuclear cells (right) were cultured in media alone (untreated), or treated with platebound rNKp30 antibodies (CLH-9 or CA1071), or control antibodies (mIgG1 or rb IgG) for 24 h and supernatants were assessed for IFN-γ by ELISA.