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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Biol Psychiatry. 2009 May 7;66(3):214–222. doi: 10.1016/j.biopsych.2009.02.033

Fig. 2.

Fig. 2

Freud-2 protein is enriched in human prefrontal cortex and hippocampus, but weakly detected in raphe. Nuclear fractions from individual samples of human prefrontal cortex (PFC), dorsal raphe (DR) and hippocampus (HP) were isolated and probed using anti-Freud-2 antiserum (Anti-Freud-2) or preimmune serum as negative control; a representative blot of three replicates is shown. Blots were reprobed for beta-actin as loading control. A major 120-kDa Freud-2 protein species was identified as a doublet. B. Detection of Freud-2 in raphe. Increasing amount of protein was loaded as indicated (30 or 40 μg) and probed using anti-Freud-2; the 120 kDa band was weakly detected in dorsal raphe tissue.