Fig. 5.

Specific binding of Freud-2 to human DRE sequences. Electrophoretic mobility shift assay (EMSA) was done using bacterially expressed purified recombinant GST-Freud-2 fusion protein (GST-hF2) or GST alone with labelled 5′or 3′DRE from the human 5-HT1A promoter. For competition, unlabelled 5′or 3′DRE (cold) were used at 100-fold molar excess. Antibody to GST (GST-Ab, 2 μl/sample) was added as indicated. A single band (arrow) was detected which was competed with excess unlabeled 5′ or 3′ DRE, indicating that Freud-2 protein binds both 5′ and 3′ DRE from human 5-HT1A promoter.