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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Environ Exp Bot. 2010 Dec 1;69(3):343–352. doi: 10.1016/j.envexpbot.2010.04.014

Fig. 4.

Fig. 4

Colonization of roots of quailbush by Bacillus pumilus ES4 detected by fluorescent in situ hybridization (FISH) using double hybridization with the specific probe BAC07 and the universal probe EUB338 and viewed by confocal laser microscopy. (A) non-inoculated roots; (B,C) root tip and elongation zone 15 days after inoculation, respectively, (D) root elongation zone 60 days after inoculation (E) root tip of the same root 60 days after inoculation, and (F) root tip and elongation zone 60 days after inoculation. Yellow colonies and yellow patches are labeled cells of Bacillus pumilus ES4 (marked with white arrows). Red colonies and red patches are labeled cells of unidentified bacteria on the roots (marked with green arrows). Because all non-inoculated roots showed the same bacterial population, only one control microphotograph is presented. RS = Root surface; RT = Root tip.