Figure 2.

(a) Priming to KLH after KLH-pulsed DCs. KLH-specific proliferation at baseline and at 30 days after control DC injection and KLH-pulsed DC injection are shown (except in D5, who demonstrated priming only at day 60). For A1–A4, data shown are for baseline and 30 days after antigen injection. For each measurement shown, 10⁵ PBMCs were incubated with 10 μg/mL KLH for 5 days. Results are expressed as [³H]TdR incorporation in counts per minute. SEM for all measurements was <20%. (b) Kinetics of KLH priming as determined by antigen-specific proliferation in a representative subject (D1). For each assay, 3 × 10⁴ or 1 × 10⁵ PBMCs per well were incubated with 1 or 10 μg/mL KLH for 5 days. Results, as shown for 10⁵ PBMCs per well, are expressed as [³H]TdR incorporation in counts per minute. (c) CD4 nature of the KLH proliferative response. KLH-specific proliferation in unseparated PBMCs was compared with that after CD4⁺ and CD8⁺ T-cell depletion by panning.