Figure 4.

Affinities of NP-specific CTL following DNA immunization. LCMV-NP CTLs were determined by using serial log dilutions of LCMV-NP H-2^d peptide on syngeneic BALB/c targets in a 5-hour ⁵¹Cr-release assay. The overall plateau release was decreased in pCMV-NP–treated groups compared with pCMV-treated controls; however, the fall-off of the curve was not shifted, indicating that there were no significant affinity differences. Decrease in CTL numbers was confirmed by pCTL analysis (Table 1). Three mice per group were injected with pCMV or pCMV-NP, according to protocol 2 in Figure 2.