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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Immunol. 2014 May 23;193(1):161–169. doi: 10.4049/jimmunol.1400381

Figure 2. Tissue location of ovarian cells with nuclear IL33 (nIL33+ cells) in mice during hCG-induced ovulation as detected by immunofluorescence.

Figure 2

A. An ovulating mature follicle shows many nIL33+ cells (red) around inner layer of its theca. Note that nIL33 staining (red) is colocalized to the cells, which express smooth muscle α-actin (SMα-actin, green). B. An enlarged view of co-localization of nIL33+ (red) with SMα-actin+ (green) cells. C. nIL33+ cells (red) in the theca of ovulating follicles also express CD31 (green). D. A group of nIL33+ cells (red) are mingled with granulosa cells of an ovulating follicle with a distance from SMα-actin+ cells (green). Note different nuclear morphology of granulosa cells and nIL33+ cells. A SMα-actin associated nIL33+ cell is also shown (arrow). Nuclei were counter stained by DAPI. Bars in B, C and D = 20 μm.