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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Immunol. 2014 May 23;193(1):161–169. doi: 10.4049/jimmunol.1400381

Figure 6. Interference with IL33 fails to block hCG-induced ovulation.

Figure 6

A and B show the results of two experiments in which IL33 antibodies were injected at −6 and 0hr relative to hCG injection, respectively. The results are expressed as ovulated eggs/mouse. C. the left panel shows SDS-PAGE for whole lysate and purified rIL33 as a 31kDa protein; the right panel is western blot detection of sera anti-rIL33 Ab from a representative rIL33 or CFA immunized mouse. Whole lysate was used as antigens. D. Summary of hCG-induced ovulation for mice immunized with rIL33 or CFA. The results are expressed as ovulated eggs/ovary. Two-tailed unpaired t test was used for statistical analysis.