Fig.3. Induction of IL-17, IL-17RA, IL-17RC, RORc and RORa mRNAs expression in PBMCs stimulated with P. acnes.
PBMCs were cultured (2–5 × 106/ml) with P. acnes sonicate (2 µg/ml). Real time PCR of IL-17, IL-17RA, IL-17RC, RORc and RORa mRNA expression was analyzed 24 hours following P. acnes stimulation. Gene expression was normalized to the housekeeping genes GAPDH and quantified by the comparative method 2−ΔΔCT. Data are representative of four independent experiments. Data represent mean ± SD (***p ≤ 0.001)