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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Invest Dermatol. 2013 Aug 7;134(2):366–373. doi: 10.1038/jid.2013.334

Fig.6. Effects of 1,25D3, 25D3 and ATRA on Th17 differentiation.

Fig.6

Human PBMCs were stimulated with P. acnes sonicate (2 µg/ml) in the presence of 1, 25D3 (10−7 M), 25D3 (10−7 M) and ATRA (10−7 M). IL-17 (a), RORa (c), RORc (d), IL-17RA (e) and IL-17RC (f) mRNA expression was analyzed 24 hours following P. acnes stimulation. Gene expression was normalized to the housekeeping genes GAPDH and quantified by the comparative method 2−ΔΔCT. Each panel is representative of three independent donors. b) PBMCs were cultured (2–5 × 106/ml) with 1,25D3, 25D3 and ATRA for one hour followed by seven days of stimulation with P. acnes (sonicate or live), IL-17 production was then measured using ELISA. Data are representative of four independent experiments. Data represent mean ± SD (***p ≤ 0.001)