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. 2014 Jul 7;9(7):e102006. doi: 10.1371/journal.pone.0102006

Table 2. Prevalence and MLST subtypes of C. andersoni from dairy cattle and beef cattle.

Collection site Dairy cattle Beef cattle
Positive no./Examined no.(%) Subtyped no./Amplified no.a MLST subtypes (n)b Positive no./Examined no. (%) Subtyped no./Amplified no.a MLST subtypes (n)b
Daqing 22/112 (19.64) 6/7 A4,A4,A4,A1 (5), A2,A4,A4,A1 (1) 11/89 (12.36) 3/3 A5,A4,A4,A1 (2), A1,A4,A4,A1 (1)
Harbin 13/83 (15.66) 4/4 A4,A4,A4,A1 (4) NE
Jiamusi NE 7/108 (6.48) 2/2 A4,A4,A4,A1 (2)
Mudanjiang 40/153 (26.14) 10/12 A4,A4,A4,A1 (6), A2,A4,A4,A1 (1), A2,A4,A2,A1 (1), A4,A4,A2,A1 (2) 13/142 (9.15) 3/4 A4,A4,A4,A1 (2), A1,A4,A4,A1 (1)
Qiqihaer 12/72 (16.67) 4/4 A4,A4,A4,A1 (4) 40/66 (60.60) 13/14 A4,A4,A4,A1 (10), A5,A4,A4,A1 (3)
Total 87/420 (20.71) 24/27 A4,A4,A4,A1 (19), A2,A4,A4,A1 (2), A2,A4,A2,A1 (1), A4,A4,A2,A1 (2) 71/405 (17.53) 21/23 A4,A4,A4,A1 (14), A5,A4,A4,A1 (5), A1,A4,A4,A1 (2)

Note: NE = not examined.

a

Subtyped no. indicating the number of C. andersoni isolates subtyped successfully by PCR at all the four loci (MS1, MS2, MS3 and MS16); Amplified no. indicating the number of C. andersoni isolates analyzed by PCR at all the four loci (MS1, MS2, MS3 and MS16).

b

The haplotypes were arranged in accordance with the order of gene loci amplified, MS1, MS2, MS3 and MS16.