Table 2. Prevalence and MLST subtypes of C. andersoni from dairy cattle and beef cattle.
Collection site | Dairy cattle | Beef cattle | ||||
Positive no./Examined no.(%) | Subtyped no./Amplified no.a | MLST subtypes (n)b | Positive no./Examined no. (%) | Subtyped no./Amplified no.a | MLST subtypes (n)b | |
Daqing | 22/112 (19.64) | 6/7 | A4,A4,A4,A1 (5), A2,A4,A4,A1 (1) | 11/89 (12.36) | 3/3 | A5,A4,A4,A1 (2), A1,A4,A4,A1 (1) |
Harbin | 13/83 (15.66) | 4/4 | A4,A4,A4,A1 (4) | NE | ||
Jiamusi | NE | 7/108 (6.48) | 2/2 | A4,A4,A4,A1 (2) | ||
Mudanjiang | 40/153 (26.14) | 10/12 | A4,A4,A4,A1 (6), A2,A4,A4,A1 (1), A2,A4,A2,A1 (1), A4,A4,A2,A1 (2) | 13/142 (9.15) | 3/4 | A4,A4,A4,A1 (2), A1,A4,A4,A1 (1) |
Qiqihaer | 12/72 (16.67) | 4/4 | A4,A4,A4,A1 (4) | 40/66 (60.60) | 13/14 | A4,A4,A4,A1 (10), A5,A4,A4,A1 (3) |
Total | 87/420 (20.71) | 24/27 | A4,A4,A4,A1 (19), A2,A4,A4,A1 (2), A2,A4,A2,A1 (1), A4,A4,A2,A1 (2) | 71/405 (17.53) | 21/23 | A4,A4,A4,A1 (14), A5,A4,A4,A1 (5), A1,A4,A4,A1 (2) |
Note: NE = not examined.
Subtyped no. indicating the number of C. andersoni isolates subtyped successfully by PCR at all the four loci (MS1, MS2, MS3 and MS16); Amplified no. indicating the number of C. andersoni isolates analyzed by PCR at all the four loci (MS1, MS2, MS3 and MS16).
The haplotypes were arranged in accordance with the order of gene loci amplified, MS1, MS2, MS3 and MS16.