Figure 8. Phagocytosis inhibition of PCI binding microspheres.

The graphs represent phagocytosis of microspheres by U937 macrophages. Either the microspheres or the macrophages were preincubated with buffer (control), 100 nM PCI or 100 nM AT3. After washing away unbound protein coculture was performed for indicated time periods at 37°C or 4°C. The phagocytosis index is calculated by the multiplication of the percent phagocytosing macrophages with the mean fluorescent intensity of ingested microspheres. The 4°C values were substracted and timelines were normalized to the control showing mean and SEM of three independent experiments, each performed in duplicates. Statistical significance was evaluated by one way ANOVA and the Dunnett's multiple comparison test. * = P<0.05.