Figure 4. Melatonin enhanced fisetin–mediated apoptosis induction.

Human MeWo cells were treated with 50 µM fisetin (F) and 1.0 mM melatonin (MT) for 48 hours. The apoptosis was determined by FACS analysis with annexin-V staining (A, B). The levels of the cleaved PARP protein (C) was analyzed by Western blot. The immunofluorescence imaging analysis was used to monitor the translocation of cyto-c in MeWo (D) and Sk-mel-28 (E). The data in (B) is present as the mean ± SD of three separate experiments. *, P<0.05, significant differences between treatment groups and DMSO control groups.