Figure 1.

Experimental flow chart. (a) AgNPs were spiked and resuspended into the prepared bacteria solution. (b) AC voltage was applied to separate and collect the bacteria in the middle region. The AgNPs can also be trapped with the bacteria aggregate via the amplified positive DEP force. After bacteria-AgNP concentration and adsorption, the Raman laser was then irradiated to the bacteria-NP aggregate separated from the blood cells for the purpose of SERS identification. (c) On-chip identification of bacteria by comparing the detected SERS spectra to the spectra library.