Table 1.

Primers used for bisulphite sequencing and MSP PC Rs.

GENE	NUCLEOTIDE SEQUENCE	PCR PRODUCT SIZE (BP)
Primer Sequences for CpGPCRs (methylation-independent)
MED15_CpG-Tag	Forward	724
	5′-CCA CTC ACT CAC CCA CCC GTA GAA AAT GTA GG-3′
	Reverse
	5′-GGG TGG GAG GTG GGA GGG AAC ACA CAA ATA AC-3′
Tag sequencing	Forward
	5′- CCA CTC ACT CAC CCA CCC-3′
	Reverse
	5′-GGG TGG GAG GTG GGA GGG-3′
Primer Sequences for MSP
MED15_MSP5′	Reverse	167
	5′-AAA AAT CCC ACA ATC CAA CCC-3′
	Forward Unmethylated
	5′-GTT TTG TGA TTG AGG YGG TGG T-3′
	Forward Methylated
	5′-GTT TTG TGA TTG AGG YGG CGG C-3′
MED15_MSP3′	Forward	172
	5′-GAT ATG GGT GGT GGG AGT TGG G-3′
	ReverseMethylated
	5′-AAT CAG ACC CTA ACC TCG CCC G-3′
MyoD	Forward	158
	5′-TGA TTA ATT TAG ATT GGG TTT AGA GAA-3′
	Reverse
	5′-CCA ACT CCA AAT CCC CTC TCT AT-3′