Skip to main content
. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Free Radic Biol Med. 2007 Apr 29;43(2):300–316. doi: 10.1016/j.freeradbiomed.2007.04.015

Fig. 3. Fluorescence properties of organelle-roGFP1.

Fig. 3

A. CF15 cells expressing cytosolic, mitochondrial, ER and cell surface roGFP1 were treated with solutions containing DTTred and H2O2 at varying ratios (final concentration 10 mM in Ringer's). 385/474 nm fluorescence ratios for each compartment were recorded and background-subtracted (● cytosol, ○ mitochondria, × surface and ▲ ER; mean of n = 6 experiments shown, respectively), B. Normalization of data in Fig. 3A to maximal reduction and oxidation of roGFP1 fluorescence ratios show redox-sensing properties of roGFP1 were very similar for each organelle roGFP1.