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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Free Radic Biol Med. 2007 Apr 29;43(2):300–316. doi: 10.1016/j.freeradbiomed.2007.04.015

Fig. 5. Dose-dependent effects of DTT and H2O2 on redox potentials in the ER, surface, cytosol and mitochondria.

Fig. 5

A. Cell surface and ER roGFP1 ratios were measured during exposure of cells to increasing [DTT] (10 to 1000 μM, additions shown by arrows). B. Background-subtracted, normalized ratios for mitochondrial and cytosolic roGFP1 were calibrated, and redox potentials were plotted against [DTT]. Data were then fit (dotted line) using redox potentials measured at 10, 50, 100, 500 and 1000 μM DTT by the equation y= a*ln(x)+y0. The intercept of this fitted line at 0 μM DTT suggests a redox potential of -217 mV in the ER and -206 mV at the cell surface. C. Mitochondrial and cytosolic roGFP1 ratios were measured during exposure of CF15 cells to increasing [H2O2] (1 to 100 μM, additions shown by arrows). D. Background-subtracted cytosolic and mitochondrial roGFP1 ratios were calibrated, and redox potentials were plotted as a function of [H2O2].