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. 1999 Aug 15;104(4):447–457. doi: 10.1172/JCI5971

Figure 1.

Figure 1

Microvessels isolated from rat epididymal fat-pads. Isolated epididymal fat-pads were digested with collagenase and homogenized as described in Methods. The purity of the isolated microvessels were verified by histochemical staining with H&E (a) and immunohistochemical staining with factor VIII antibody (b). Immunoblotting with antibody against vascular α-SMA was used to compare the quality of isolated microvessels from lean and obese rats (c).