Figure 7.

IRS-2–associated PI 3-kinase activities in the aorta and liver after euglycemic hyperinsulinemic clamp. Animals were infused with saline (control) or insulin (10 mU/kg/min) for 1 hour during a euglycemic clamp as described in Methods. IRS-2 associated PI 3-kinase in the aorta, and liver was immunoprecipitated with αIRS-2 antibody. The kinase activities were measured in the presence of phosphatidylinositol and [³²P]ATP, and the lipid products were separated by TLC (top panels). The spots that comigrated with PIP standard were quantified by a PhosphorImager. Data (mean ± SD; n = 5) are expressed relative to control, assigning a value of 100% to the control mean. *P < 0.05, insulin lean vs. insulin obese.