Figure 8.

Effects of insulin on tyrosine phosphorylation of MAP kinase (ERK-1/2) in microvessels ex vivo. Isolated microvessels were incubated without or with insulin (2 or 100 nM) in DMEM (0.1% BSA) for 10 minutes at 37°C. Fifty micrograms of protein of each sample was separated by SDS-PAGE and immunoblotted with antibody specific for tyrosine-phosphorylated ERK-1/2. Stripped membranes were reblotted with polyclonal antibody against ERK-1/2 proteins. Data (mean ± SD; n = 6) are expressed relative to control, assigning a value of 100% to lean control mean.