Fig. 2.

Effect of high Ca²⁺ medium on relative luciferase activities of SPINK5 putative promoters. pGLSP−676/−532, pGLSP−318/−1 and pGLSP−318/−146 were transfected to normal HuMedia-KG2-, low Ca²⁺ medium-, and high Ca²⁺ medium-cultured NHEK. Each column represents the mean of three independent experiments, each done in triplicate; bars, ± SD. No significant difference was observed in any of pGLSP−932/−1, pGLSP−676/−1 and pGLSP−318/−1 among different culture media (One-way ANOVA and post hoc test using Tukey’s HSD test)