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Multiplexed magneto-enrichment immunoassay. After binding of the biotinylated anti-PfHRP2 and anti-aldolase IgG antibodies to the mixed antigens in human plasma, the universal streptavidin gold reagent was added, followed by magnetic enrichment and immunochromatographic readout. The top panel shows the respective concentrations of biomarkers in the original 500 μL sample.
