Table 1.

Specific activities and kinetics parameters of the wildtype and evolved Rubisco

Rubisco	Specific carboxylation activity (U/mg)	$k_{\text{cat}}^{\text{C}}$ (s−1)	$K_{\text{M}}^{\text{C}}$ (μmol/L)	$k_{\text{cat}}^{\text{C}}/K_{\text{M}}^{\text{C}}$ [s−1 (mmol/L)−1]	$K_{\text{M}}^{\text{RuBP}}$ (μmol/L)
WT	0.91 ± 0.04	6.7 ± 0.6	131.5 ± 4.6	51	51.8 ± 8.4
M6-5	1.68 ± 0.14	14.6 ± 1.1	198.0 ± 12.4	74	49.4 ± 8.7

±: standard deviations of five to six independent experiments including expression, purification and activity assay