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. 2014 Jun 21;15(1):507. doi: 10.1186/1471-2164-15-507

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© Bao et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Tissue-specific expressions of trypsin-like genes. Total RNA was extracted from the fat body, midgut, salivary gland, ovary, male reproductive system and carcass (the remaining body) of N. lugens individuals, and used for the expression analysis of trypsin-like genes using qRT-PCR. The relative expression levels of each trypsin-like gene in each tissue were normalized using N. lugens 18 s rRNA threshold cycle (Ct) values. Three biological replications were conducted and the ΔΔCt method was used to measure the relative transcript levels in tissues. Results of triplicate experiments are shown with the standard deviations. The asterisk (**) indicates statistical significance at p < 0.01. Sg, salivary gland; Mg, midgut; MRS, male reproductive system; Ov, ovary; Fb, fat body; Cc, carcass.