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. 2014 Jun 21;15(1):507. doi: 10.1186/1471-2164-15-507

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© Bao et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analysis of clotting factor C like genes in insects. (A) Schematic representation of clotting factor C-like proteins from insect species. Red bars, green hexagons, orange circle and blue oblongs indicate the putative signal peptide sequence, LDLa domain, CCP domain and serine protease domain with the catalytic triad, respectively. The size bar indicates the amino acid residues of the deduced proteins. (B) Phylogenetic analysis of insect clotting factor C like genes. The phylogenetic tree was constructed based on the deduced amino acid sequences of clotting factor C like genes by maximum likelihood, using the program Mega 5.05 (http://www.megasoftware.net/). The Jones-Taylor-Thornton (JTT) model for amino acid substitution was used, while a test of phylogeny was carried out using the bootstrap analysis with 1000 replications. The GenBank accession numbers for the sequences are as follows: Nilaparvata lugens (KJ512060); Camponotus floridanus (EFN71201); Acyrthosiphon pisum (XP_001944706); Apis mellifera (XP_001120594); Bombus terrestris (XP_003403297); Danaus plexippus (EHJ70705); Bombyx mori (AFK93534); Culex quinquefasciatus (XP_001864236); Tribolium castaneum (XP_967486); Aedes aegypti (XP_001655952); Manduca sexta (AAR29602); Megachile rotundata (XP_003707203).