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. 2014 Jun 21;15(1):507. doi: 10.1186/1471-2164-15-507

Table 1.

The genomic prediction of N. lugens serine protease and serine protease homologs

Predicted gene GenBank ID Locus Size (aa) Exon Orientation HDS Domains/Motifs Best match Similarity E-value Cover
Trypsin-like serine protease
Trypsin 1 KJ512112 scaffold574 334 7 + active Signal P P. h.corporis 47% 1e-28 95%
Trypsin 2 KJ512113 scaffold261 360 7 - active Signal P P. h.corporis 79% 5e-137 78%
Trypsin 3 KJ512114 scaffold998 299 6 - active P. h.corporis 62% 4e-91 99%
Trypsin 4 KJ512115 scaffold106 795 5 - active Signal P P. h.corporis 84% 4e-178 41%
Trypsin 5 KJ512116 scaffold126 347 3 - active Signal P P. h.corporis 69% 1e-114 90%
Trypsin 6 KJ512117 scaffold126 360 7 + active Signal P P. h.corporis 79% 5e-137 78%
Trypsin 7 KJ512118 scaffold64 393 4 - active Signal P P. h.corporis 89% 0 84%
Trypsin 8 KJ512119 scaffold260 323 5 + active Signal P P. h.corporis 65% 3e-95 91%
Trypsin 9 KJ512120 scaffold189 318 12 - active Signal P N.lugens 98% 0 100%
Trypsin 10 KJ512121 scaffold707 303 8 - active Signal P N.lugens 54% 4e-58 79%
Trypsin 11 KJ512122 scaffold1712 290 5 + active Signal P C.quinquefasciatus 54% 2e-46 92%
Trypsin 12 KJ512123 scaffold172 295 12 - active Signal P A.mellifera 53% 6e-44 90%
Trypsin 13 KJ512124 scaffold6559 423 3 - active Signal P D.pteronyssinus 59% 9e-50 77%
Trypsin 14 KJ512125 scaffold6559 287 1 - active Signal P D.pteronyssinus 58% 1e-53 79%
Trypsin 15 KJ512126 scaffold6559 731 7 - active Signal P D.pteronyssinus 61% 8e-66 99%
Trypsin 16 KJ512127 scaffold6559 262 2 - active Signal P C.capitata 51% 2e-27 96%
Trypsin 17 KJ512128 scaffold50 318 6 + active Signal P C.felis 59% 4e-64 89%
Trypsin 18 KJ512129 scaffold327 292 5 - active Signal P A.mellifera 48% 5e-25 79%
Trypsin 19 KJ512130 scaffold126 511 7 - active Signal P T.castaneum 68% 0 94%
Trypsin 20 KJ512131 scaffold577 294 1 + active Signal P D.melanogaster 48% 5e-32 87%
Trypsin 21 KJ512132 scaffold1720 272 4 - active H. saltator 48% 4e-20 70%
Trypsin 22 KJ512133 scaffold1722 747 14 + active A.pisum 54% 2e-52 75%
Trypsin 23 KJ512134 scaffold299 260 6 - inactive Signal P N.vitripennis 45% 4e-25 85%
Trypsin 24 KJ512135 scaffold299 290 5 - inactive Signal P N.lugens 98% 0 94%
Trypsin 25 KJ512136 scaffold299 300 7 - Inactive Signal P N.lugens 56% 4e-49 82%
Trypsin 26 KJ512137 scaffold299 292 5 - Inactive Signal P N.lugens 50% 4e-53 84%
Trypsin 27 KJ512138 scaffold299 266 6 - inactive Signal P N.vitripennis 45% 7e-25 97%
Trypsin 28 KJ512139 scaffold601 298 1 - inactive Signal P T.castaneum 48% 3e-23 74%
Trypsin 29 KJ512140 scaffold682 325 6 + inactive Signal P P. h.corporis 66% 3e-106 95%
Trypsin 30 KJ512141 scaffold2065 281 6 + inactive Signal P D. yakuba 55% 6e-34 79%
Trypsin 31 KJ512142 scaffold4540 247 4 - inactive P. h.corporis 53% 2e-31 91%
Clotting serine proteases
Clotting factor C like KJ512060 scaffold437 553 2 + active CCP C.biroi 52% 1e-108 96%
Clotting factor B like KJ512061 scaffold867 321 7 - inactive Signal P B.impatiens 53% 1e-52 82%
Proclotting enzyme 1 KC355213 scaffold424 397 7 + active Clip A.pisum 56% 4e-91 91%
Proclotting enzyme 2 KC355214 scaffold424 376 12 - active Clip A.pisum 55% 6e-89 91%
Proclotting enzyme 3 KC355215 scaffold1854 460 9 - active Clip C.floridanus 66% 4e-73 90%
Serine protease nudel
Serine protease nudel like KJ512077 scaffold771 683 11 - active LDLA A.pisum 47% 2e-86 97%
Serine protease gd
Serine protease gd like KJ512078 scaffold50 441 10 + active Signal P A.florea 57% 3e-96 94%
Serine protease snake
Serine protease snake 1 KC355219 scaffold407 363 7 + active Clip A.pisum 54% 3e-74 89%
Serine protease snake 2 KC355220 scaffold183 406 5 - active Clip A.pisum 50% 7e-71 99%
Serine protease snake 3 KC355221 scaffold183 406 7 - active Clip A.pisum 47% 1e-71 99%
Serine protease snake 4 KC355222 scaffold3538 546 7 + active Clip T.castaneum 58% 3e-65 95%
Serine protease snake 5 KC355223 scaffold407 358 8 - inactive Clip A.pisum 41% 8e-31 89%
Serine protease snake 6 KC355224 scaffold407 374 7 - inactive Clip A.pisum 45% 1e-33 93%
Serine protease snake 7 KC355225 scaffold407 362 7 - active Clip A.pisum 53% 5e-70 85%
Serine protease snake 8 KJ512098 scaffold407 389 7 - active A.pisum 60% 1e-74 86%
Serine protease snake 9 KJ512099 scaffold407 489 6 + active Signal P A.pisum 57% 4e-70 56%
Serine protease snake 10 KJ512100 scaffold407 367 7 - active Signal P A.pisum 56% 3e-66 80%
Serine protease snake 11 KJ512101 scaffold4413 489 7 - active Signal P A.pisum 60% 2e-69 56%
Serine protease snake 12 KJ512102 scaffold4413 389 6 + active A.pisum 55% 1e-74 86%
Serine protease easter
Serine protease easter 1 KJ512062 scaffold258 289 3 + active H. saltator 45% 3e-31 97%
Serine protease easter 2 KJ512063 scaffold258 319 6 + active Signal P H. saltator 52% 2e-45 98%
Serine protease easter 3 KJ512064 scaffold258 397 7 + active N.vitripennis 51% 2e-27 61%
Serine protease easter 4 KJ512065 scaffold258 407 7 - active Signal P H. saltator 49% 1e-41 76%
Serine protease easter 5 KJ512066 scaffold258 334 5 - inactive Signal P H. saltator 45% 5e-19 80%
Serine protease easter 6 KJ512067 scaffold258 330 6 - inactive Signal P H. saltator 45% 4e-30 92%
Serine protease easter 7 KJ512068 scaffold574 315 7 + active Signal P H. saltator 52% 8e-50 92%
Serine protease easter 8 KJ512069 scaffold574 323 5 - active Signal P H. saltator 48% 4e-35 98%
Serine protease easter 9 KJ512070 scaffold1012 318 6 + inactive Signal P A.aegypti 43% 9e-17 80%
Serine protease easter 10 KJ512071 scaffold1012 356 6 + inactive Signal P A.aegypti 43% 2e-17 90%
Serine protease easter 11 KJ512072 scaffold1012 322 7 + active Signal P H. saltator 50% 5e-41 92%
Serine protease easter 12 KJ512073 scaffold1121 334 6 - active Signal P H. saltator 62% 4e-72 85%
Serine protease easter 13 KJ512074 scaffold236 310 10 - active Signal P H. saltator 52% 2e-43 94%
Serine protease easter 14 KJ512075 scaffold4778 307 8 - active Signal P H. saltator 48% 2e-32 96%
Serine protease easter 15 KJ512076 scaffold4872 340 4 + inactive Signal P A.mellifera 45% 5e-20 91%
Serine protease stubble
Serine protease stubble like 1 KJ512103 scaffold126 324 7 - active Signal P M. rotundata 78% 9e-148 99%
Serine protease stubble like 2 KJ512104 scaffold126 371 7 + active Signal P A.echinatior 55% 3e-32 70%
Serine protease stubble like 3 KJ512105 scaffold223 398 7 + active Signal P B. mori 64% 8e-119 86%
Serine protease stubble like 4 KJ512106 scaffold115 368 7 - active H. saltator 79% 0 100%
Serine protease stubble like 5 KJ512107 scaffold886 467 5 + inactive M rotundata 62% 4e-129 84%
Serine protease
Serine protease 1 KJ512079 scaffold63 849 4 + inactive H. saltator 71% 2e-28 26%
Serine protease 2 KJ512080 scaffold1489 324 3 - active Signal P B.mori 52% 4e-31 85%
Serine protease 3 KJ512081 scaffold219 743 11 + active MADF A. pisum 93% 1e-141 64%
Serine protease 4 KJ512082 scaffold1220 550 4 + active CUB A. pisum 57% 5e-137 88%
Serine protease 5 KJ512083 scaffold1208 492 10 + active CUB A.pisum 74% 3e-93 83%
Serine protease 6 KJ512084 scaffold1877 258 4 + inactive Signal P C.formosanus 47% 2e-20 75%
Serine protease 7 KJ512085 scaffold185 332 7 + active Signal P D.mojavensis 55% 1e-49 74%
Serine protease 8 KJ512086 scaffold762 346 6 - active T.castaneum 65% 2e-83 88%
Serine protease HP21 KJ512089 scaffold2889 307 2 - inactive Signal P T.castaneum 52% 1e-33 86%
Prophenoloxidase activating factor 1 KJ512096 scaffold66 390 7 - inactive Signal P T.molitor 59% 2e-120 100%
Prophenoloxidase activating factor 2 KJ512097 scaffold66 395 6 - inactive T.molitor 69% 3e-123 83%
Hemolymph protease 1 KJ512090 scaffold972 314 1 - inactive Signal P T.castaneum 47% 1e-32 86%
Hemolymph protease 2 KJ512091 scaffold236 314 6 - inactive C.quinquefasciatus 43% 7e-16 82%
Serine protease SP24D KJ512092 scaffold2296 310 6 + active Signal P C.quinquefasciatus 48% 1e-18 78%
Serine protease P69 KJ512093 scaffold598 708 8 + active Signal P T.castaneum 69% 7e-84 61%
Serine protease HTRA2 KJ512094 scaffold552 420 7 + active PDZ N.vitripennis 70% 5e-133 91%
Transmembrane serine protease
Serine protease 11 KJ512108 scaffold967 919 18 - active FRI & SR T.castaneum 72% 0 86%
Ovochymase 1 KJ512109 scaffold305 322 7 + active TM A.pisum 77% 3e-124 87%
Ovochymase 2 KJ512110 scaffold126 360 7 - active TM T.castaneum 87% 3e-147 68%
Ovarian serine protease KJ512111 scaffold498 1334 9 + active TM T.castaneum 71% 2e-97 47%

Table 1 Identification of SP and SPH genes in the N. lugens genome. The SP and SPH sequences were obtained from the N. lugens transcriptome databases and genomic sequences, which were confirmed using the tBLASTX algorithm with a cut-off E-value of 10-10. The genomic organization of exons and introns of the SP and SPH genes was predicted based on the mRNA-genome alignments at the NCBI spideyweb (http://www.ncbi.nlm.nih.gov/spidey/spideyweb.cgi). Locus, size and orientation indicate the location on scaffolds, predicted amino acids (aa) and the transcription orientation of the genes. HDS refers to the presence or absence of His, Asp and Ser residues in the catalytic triad, implying active or inactive proteases. A.pisum, Acyrthosiphon pisum; T. castaneum, Tribolium castaneum; P. h. corporis, Pediculus humanus corporis; B. mori, Bombyx mori; C.quinquefasciatus, Culex quinquefasciatus; C. capitata, Ceratitis capitata; H. saltator, Harpegnathos saltator; A. mellifera, Apis mellifera; M. rotundata, Megachile rotundata; A.echinatior, Acromyrmex echinatior; N. lugens, Nilaparvata lugens; C. felis, Ctenocephalides felis; D. pteronyssinus, Dermatophagoides pteronyssinus; D. melanogaster, Drosophila melanogaster; D.mojavensis, Drosophila mojavensis; D. yakuba, Drosophila yakuba; C. floridanus, Camponotus floridanus; A. florea, Apis florea; N.vitripennis, Nasonia vitripennis; A. aegypti, Aedes aegypti; B. impatiens, Bombus impatiens; T. molitor, Tenebrio molitor; C. formosanus, Coptotermes formosanus; C. biroi, Cerapachys biroi.