Fig 5.

Effect of cholesterol enriched diet and vitamin E treatment on MMP-9 protein and mRNA expressions in rabbit aorta. mRNA expression in aortic tissue of each animal was measured by quantitative RT-PCR, normalized to GAPDH. Relative mRNA expression in rabbit aorta for MMP-9 (A). Protein expression analyzed by Western blotting with densitometric analysis of protein bands and relative ratios were quantified and normalized relative to GAPDH against MMP-9 (B) antibody. Data denote mean ± S.D. *p < 0.05 vs. cholesterol group, (n = 5).